diff --git a/Lib/test/test_io/test_memoryio.py b/Lib/test/test_io/test_memoryio.py
index c82acceffb09c8b..f70a589f45d47a9 100644
--- a/Lib/test/test_io/test_memoryio.py
+++ b/Lib/test/test_io/test_memoryio.py
@@ -1004,6 +1004,13 @@ class CStringIOTest(PyStringIOTest):
     ioclass = io.StringIO
     UnsupportedOperation = io.UnsupportedOperation
 
+    def test_truncate_large_size(self):
+        # Sizes above 2**31 - 1 must work where C long is 32-bit.
+        if sys.maxsize > 2**31:
+            stream = self.ioclass("abc")
+            self.assertEqual(stream.truncate(2**31), 2**31)
+            self.assertEqual(stream.getvalue(), "abc")
+
     # XXX: For the Python version of io.StringIO, this is highly
     # dependent on the encoding used for the underlying buffer.
     def test_widechar(self):
diff --git a/Misc/NEWS.d/next/Library/2026-10-27-15-27-32.gh-issue-160781.kARuHu.rst b/Misc/NEWS.d/next/Library/2026-10-27-15-27-32.gh-issue-160781.kARuHu.rst
new file mode 100644
index 000000000000000..1f1e25d8b461b4e
--- /dev/null
+++ b/Misc/NEWS.d/next/Library/2026-10-27-15-27-32.gh-issue-160781.kARuHu.rst
@@ -0,0 +1,2 @@
+Fix :class:`io.StringIO` implementation of :meth:`io.IOBase.truncate`
+rejecting sizes larger than ``2**31 - 1`` on Windows.
diff --git a/Modules/_io/stringio.c b/Modules/_io/stringio.c
index b8601383ad0a26f..08a3f7e16e4d8a3 100644
--- a/Modules/_io/stringio.c
+++ b/Modules/_io/stringio.c
@@ -469,7 +469,7 @@ _io_StringIO_truncate_impl(stringio *self, PyObject *pos)
         size = self->pos;
     }
     else {
-        size = PyLong_AsLong(pos);
+        size = PyNumber_AsSsize_t(pos, PyExc_OverflowError);
         if (size == -1 && PyErr_Occurred()) {
             return NULL;
         }